Biomimetic Thioesters as Probes for Enzymatic Assembly Lines: Synthesis, Applications, and Challenges
نویسندگان
چکیده
منابع مشابه
Facile enzymatic synthesis of fatty acylcoenzyme A thioesters.
The fatty acid:CoA ligase (acyl-CoA synthetase, EC 6.2.1.3) of rat liver microsomes was solubilized with Triton X-100 and bound to Matrex Gel Red A. Fatty acid:CoA ligase immobilized on Matrex Gel Red A was active and proved useful for the synthesis of fatty acyl-CoA thioesters. The immobilized activity was characterized by a 3-fold higher apparent Km for ATP than the soluble activity, similar ...
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Self-assembly and applications of biomimetic and bioactive peptide- amphiphiles
Peptide-amphiphiles are amphiphilic structures with a hydrophilic peptide headgroup that incorporates a bioactive sequence and has the potential to form distinct structures, and a hydrophobic tail that serves to align the headgroup, drive self-assembly, and induce secondary and tertiary conformations. In this paper we review the different self-assembled structures of peptide-amphiphiles that ra...
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ژورنال
عنوان ژورنال: Cell Chemical Biology
سال: 2016
ISSN: 2451-9456
DOI: 10.1016/j.chembiol.2016.08.014